EverGreen Universal qPCR Master Mix (5mL)

SAR 1288 VAT included

2X Master Mix for dye-based real-time PCR.

SKU: PCR5505 Category:

Description

2X Master Mix for dye-based real-time PCR. Sufficient for 500 reactions of 20µL. Research use only.

This product contains all components needed for an efficient real-time qPCR reaction: a robust DNA polymerase, pure dNTPs, stabilizers, enhancers, and the intercalating dye. You only need to add the primers and template.

EverGreen yields consistent results over a wide range of template concentrations. Figure1 shows the amplification curves of the human β2M gene in a 4-fold serial dilution of a cDNA template, giving a qPCR efficiency of 103% (Figure2).

 

Figure1Figure1.-Amplification-Plot-B2M

 

Figure2Figure2.-qPCR-Efficiency-scaled

 

Manual

Manual

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